| Graham Chapman | Wise Man #2/Brian Cohen/Biggus Dickus |
| Terry Gilliam | Man Even Further Forward/Revolutionary/Jailer/Blood & Thunder Prophet/Frank/Audience Member/Crucifee |
| Terry Jones | Mandy Cohen/Colin/Simon the Holy Man/Bob Hoskins/Saintly Passer-by/Alarmed Crucifixion Assistant |
| Terence Bayler | Gregory |
| Kenneth Colley | Jesus |
| Charles McKeown | False Prophet/Blind Man/Giggling Guard/Stig |
| Gwen Taylor | Mrs. Big Nose |
| Chris Langham | Alfonso/Giggling Guard |
| Bernard McKenna | Parvus/Official Stoners Helper/Giggling Guard |
| George Harrison | Mr. Papadopolous |
| John Cleese | Wise Man #1/Reg/Jewish Official/Centurion/Deadly Dirk/Arthur |
| Eric Idle | Mr. Cheeky/Stan (Loretta)/Harry the Haggler/Culprit Woman/Warris/Intensely Dull Youth/Jailer's Assistant/Otto/Lead Singer Crucifee |
| Michael Palin | Wise Man #3/Mr. Big Nose/Francis/Mrs. A/Ex-Leper/Announcer/Ben/Pontius Pilate/Boring Prophet/Eddie/Shoe Follower/Nisus Wettus |
| Carol Cleveland | Mrs. Gregory |
| Neil Innes | A weedy Samaritan |
| John Young | Matthias, Son of Deuteronomy of Gath |
| Sue Jones-Davies | Judith |
| John Case | Pilate's Wife |
| Andrew MacLachlan | Another Official Stoners Helper/Giggling Guard |
| Spike Milligan | Spike |
| Charles Knode | Passer-by |