| Quincy Jones | Himself - Host (segment "Rhapsody in Blue") |
| Bette Midler | Herself - Hostess (segment "Piano Concerto No. 2, Allegro, Opus 102") |
| James Earl Jones | Himself - Host (segment "Carnival of the Animals") |
| Penn Jillette | Himself |
| Teller | Himself - Host (segment "The Sorcerer's Apprentice") |
| Leopold Stokowski | Himself - Conductor (segment "The Sorcerer's Apprentice") |
| Kathleen Battle | Featured Soprano (segment "Pomp and Circumstance") |
| Itzhak Perlman | Himself - Host (segment "Pines of Rome") |
| James Levine | Himself - Host (segment "Pomp and Circumstance"), Conductor (Chicago Symphony Orchestra) |
| Wayne Allwine | Mickey Mouse (segment "Pomp and Circumstance") |
| Russi Taylor | Daisy Duck (segment "Pomp and Circumstance") |
| Ga??tan Brizzi | Animator |
| Yefim Bronfman | Pianist (segment "Piano Concerto No. 2, Allegro, Opus 102") |
| Eric Goldberg | Animator (segment "Carnival of the Animals") |
| Ralph Grierson | Pianist (segment "Rhapsody in Blue") |
| Steve Martin | Himself - Introductory Host |
| Teller | Himself - Host (segment "The Sorcerer's Apprentice") |
| Angela Lansbury | Herself - Hostess (segment "Firebird Suite - 1919 Version") |
| Tony Anselmo | Donald Duck (segment "Pomp and Circumstance") |
| Deems Taylor | Himself - Host (segment "Fantasia Introduction") |
| Paul Brizzi | Animator |
| Hendel Butoy | Animator |