- Available Subtitles: Spanish, French
- Available Audio Tracks: English (Dolby Digital 5.1 EX), Spanish (Dolby Digital 5.1 EX), French (Unknown Format)
- Disc One: Filmmakers' World
- Widescreen presentation of film with introduction (1.78:1, includes French & Spanish subtitles)
- Visual commentary with deleted scenes & recording sessions
- "Making Nemo" documentary
- "The Art of Nemo" narrated by the artists
- Virtual aquariums
- Disc Two: Family Fun
- Reframed full screen presentation of film with introduction (1.33:1, includes French & Spanish tracks)
- "Exploring the Reef" a new short with Jean-Michel Cousteau and your Nemo friends
- Pixar short Knick Knack with commentary
- A peek at the next Pixar film, The Incredibles
- Fisharades game
- Read-along storytime
- Learning fun with Mr. Ray's encyclopedia
- Behind the scenes: A tour of the Pixar Studios, character interviews, publicity
- More virtual aquariums
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| Alexander Gould | Nemo | | Willem Dafoe | Gill | | Brad Garrett | Bloat | | Austin Pendleton | Gurgle | | Andrew Stanton | Crush | | Albert Brooks | Marlin | | Allison Janney | Peach | | Vicki Lewis | Deb/Flo | | Geoffrey Rush | Nigel | | Elizabeth Perkins | Coral | | Bob Peterson | Mr. Ray | | Eric Bana | Anchor | | Bill Hunter | Dentist | | Jordan Ranft | Tad | | Erik Per Sullivan | Sheldon | | Ellen DeGeneres | Dory | | Stephen Root | Bubbles | | Joe Ranft | Jacques | | Andrew Stanton | Crush | | Nicholas Bird | Squirt | | Barry Humphries | Bruce | | Bruce Spence | Chum | | LuLu Ebeling | Darla | | Erica Beck | Pearl | | John Ratzenberger | Fish School |
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